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US · guidance

CMS SOM App. C, Tag D5457

§493.1256 Standard: Control procedures

activein force · 2026-07-22 – presentas-observed

(d)(4) For thin layer chromatography--

(d)(4)(i) Spot each plate or card, as applicable, with a calibrator containing all

known substances or drug groups, as appropriate, which are identified by thin layer

chromatography and reported by the laboratory; and

(d)(4)(ii) Include at least one control material on each plate or card, as applicable,

which must be processed through each step of patient testing, including extraction

processes.

Interpretive Guidelines §493.1256(d)(4)

For qualitative urine drug screens performed by thin layer chromatography, a negative

control is not required. However, a control containing one or more drugs representative

of each drug group reported (e.g., tricyclic antidepressants, barbiturates) that goes

through each test phase (including the extraction process) is required.

History

Rev. 233; Issued: 09-12-25; Effective: 09-12-25; Implementation: 09-12-25

Provenance

Source
cms.gov
Retrieved
2026-07-22
Edition
som-2026-07-22
Content hash
6df47144a8f9d9243c04dcf624428849caccfad63fa279e0f8fb1aaed5963c66
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