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US · guidance

CMS Pub. 100-03, ch. 1, § 190.9

Serologic Testing for Acquired Immunodeficiency Syndrome

activein force · 2026-08-25 – presentas-observed

(AIDS)

(Rev. 1, 10-03-03)

CIM 50-52

Serologic testing is employed to detect antibodies to the AIDS virus which is currently

identified by the term “human immunodeficiency virus (HIV).” The virus originally was

named “human T-cell lymphotropic virus, type III (HTLV-III),” a term that remains in

common usage.

Antibodies may be detected by a variety of immunoassay techniques, the most common

being an enzyme-linked immunosorbent assay (ELISA). When an assay is reactive on

initial testing, it should be repeated on the same specimen. A more specific test,

(Western blot, immunofluorescent assay) is usually performed following repeatedly

reactive ELISA results.

These tests may be covered when performed to help determine a diagnosis for

symptomatic patients. They are not covered when furnished as part of a screening

program for asymptomatic persons.

NOTE: Two enzyme-linked immunosorbent assay (ELISA) tests that were conducted on

the same specimen must both be positive before Medicare will cover the Western blot

test.

History

(Rev. 1, 10-03-03)

Provenance

Source
cms.gov
Retrieved
2026-08-25
Edition
iom-2026-08-25
Content hash
f71bbc9051210a49d7e9a024858ed227de1e86ad87c86b201feab846e494ff52
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